Protective Effect of Aerobic Training along with Resveratrol on Mitochondrial Dynamics of Cardiac Myocytes in Animal Model of Non-alcoholic Fatty Liver Disease

  Background & objectives: Non-alcoholic fatty liver disease (NAFLD) is associated with mitochondrial dysfunction. The aim of the present study was to examine the effect of aerobic training along with resveratrol on cardiac expression of OPA1 and DRP1 in NAFLD male rats. Methods: In this experimental study, forty eight male Wistar rats were classified into two groups: NAFLD (n=40) and Control-Normal (CN), (n=8). NAFLD was induced in rats with high fat diet and then subdivided into five subgroups, including patient (NAFLD), sham (SHAM), exercise-patient (TRNAF), supplement-patient (SUPNAF) and exercise-supplement-patient (TRSUPNAF). Training groups performed a running program on a motor-driven treadmill for eight weeks. Resveratrol (20 mg/kg) was injected into the SUPNAF and TRSUPNAF groups. Forty eight hours after the last training session, rats were anesthetized; hearts were excised and immediately frozen in liquid nitrogen and stored at -80°C for measuring the indexes. Statistical analysis was performed using a one way analysis of variance, and Statistical significance was accepted at p≤ 0.05. Result: The results indicated that the induction of NAFLD decreased OPA1 expression (p=0.001) and increased DRP1 expression (p=0.001) compared to CN. Also, in SUPNAFLD and TRSUPNAF groups, OPA1 (p=0.014 and p=0.001 respectively) increased and DRP1 (p=0.043 and p=0.007 respectively) decreased significantly compared to the NAFLD group. Conclusion: It seems that exercise training and resveratrol alone or in combination by increasing OPA1 and decreasing DRP1 myocytes modulates heart damage in NAFLD patients.